BBa_K112115

BBa_K112115 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112115
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bing Xia
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:14

a~Cre



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_K112115_sequence (Version 1)

    Description

    Cre recombinase gene, with a spacer for the rbs prepended.

    This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    BBb Standard Description Page

    Source


    
PCR mv025/mv002 on pBca9145-Bca1010    (1067bp, EcoRI/BamHI/DpnI)
    
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
    
Product is pBca1256-K112115
    


    
mv025	Forward Biobricking of a~Cre!
    
ccataGAATTCatgAGATCTttaaatgtccaatttactgaccgtacacc
    
mv002	Reverse Biobricking of 
cgttaGGATCCttaatcgccatcttccagc


    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K112115/1
     

    Attachments

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