BBa_K112121

BBa_K112121 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112121

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Madhvi Venkatesh

Date created: 2008-10-29 12:00:00

Date modified: 2015-05-08 01:09:14

BglII endonuclease with ribosome binding site

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K112121_sequence (Version 1)

Description

BglII nuclease with ribosome binding site and stop codon

Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.
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PCR mv032/mv010 on pBca9145-Bca1147    (722bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112121



mv032	Forward Biobricking of rbs.r.BglII!

cgataGAATTCatgAGATCTtggaaggagacactcataatgaagattgatataacggactataaccatgc

mv010	Reverse Biobricking of r.BglII!

cgttaGGATCCttaatatgtcacgattgttcctc

Source

PCR mv032/mv010 on pBca9145-Bca1147    (722bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112121

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112121/1

Member of these Collections

• //function/dna
• iGEM Parts Registry