BBa_K1122001

BBa_K1122001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1122001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lina Gasiūnaitė
Date created: 2013-08-12 11:00:00
Date modified: 2015-05-08 01:09:14

GFP (BBa_E0040) under the control of lac promoter



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1122001_sequence (Version 1)

Description

Plac-lacZ???α + Linker1 based on lacZ with RBS and a start codon + GFP (BBa_E0040)

Notes

The assembly of lac promoter, Linker1 with RBS and a start codon upstream the GFP sequence and GFP is contained in pSB1C3 vector.

Source

GFP is based on the BioBrick BBa_E0040, lac promoter comes from genomic sequence of E. coli.

Sequence Annotation Location Component / Role(s)
lac promoter with lacZ
stop
start
linker1 based on lacZ
start
BBa_E0040
stop
rbs
1,382
380,382
159,161
383,427
429,431
429,1151
1149,1151
418,421
promoter feature/promoter
stop_codon feature/stop
start_codon feature/start
sequence_feature feature/misc
feature/start start_codon
CDS feature/cds
feature/stop stop_codon
feature/rbs ribosome_entry_site
igem#experience
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synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
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synbiohub#topLevel
BBa_K1122001/1