Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aron Lau
Date created: 2008-10-20 11:00:00
Date modified: 2015-05-08 01:09:16
{a~lambda holin!}
adheres to Berkeley standard
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K112310_sequence (Version 1) |
Description
lambda holin ready for rbs addition and has stop codon in BBb format.More information about the BBb Format is available at:
BBb Standard Description Page
Notes
PCR al0011/al0008 on pSB3C6-Bjh1502 (353bp, EcoRI/BamHI/DpnI)
Sub into pBca1256 (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112310
al0011 Forward Biobricking of lambda holin with spacer
CCATAGAATTCatgAGATCTgaagatgccagaaaaacatgacctg
al0008 Reverse Biobricking of lambda holin with spacer
CGttaGGATCCttattgatttctaccatcttctactccggc