BBa_K112319

BBa_K112319 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112319

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aron Lau

Date created: 2008-10-21 11:00:00

Date modified: 2015-05-08 01:09:16

{< ftsQ promoter>} in BBb format

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K112319_sequence (Version 1)

Description

ftsQ promoter is a promoter that has level of expression are inversely proportional to the growth rate. For more information, refer to the following link. http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=208112&blobtype=pdf This part is actually in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

PCR al0019/al0024 on MG1655 (404bp, gp = A)

PCR al0023/al0023 on MG1655 (86bp, gp=B)



PCR al0019/al0020 on A+B     (465bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112319



al0019	Forward Biobricking of ftsQ promoter

CCATAGAATTCatgAGATCTttgattgaaaaatggctaagtgggccgg

al0020	Reverse Biobricking of ftsQ promoter

CGttaGGATCCcctcttcttcgctgtttcgc

al0023 Forward Removing of EcoRI

ggtagtacgaattGtggaactggcg

al0024 Reverse Removing of EcoRI

cgccagttccaCaattcgtactacc

Source

MG1655

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112319/1

Member of these Collections

• iGEM Parts Registry