HA tag

BBa_K112506 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112506

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sherine Cheung

Date created: 2008-10-21 11:00:00

Date modified: 2015-05-08 01:09:18

< HA > in BBb

Details

• Types: DnaRegion
• Roles: Tag; tag
• Sequences: BBa_K112506_sequence (Version 1)

Description

This is an HA tag without a start or a stop codon. It is in BBb format.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:


BBb Standard Description Page

Notes

N/A

Source

Construction of {} basic part K112506



Wobble PCR of cc001/cc010                  ( 51 bp, EcoRI/BamHI)

Sub into pBca1256                          (EcoRI/BamHI, 2227+ 910,L)

Product is pBca1256-K112506



cc001             Forward Biobricking of {} 

CgATAgaattcATGagatcttacccatacgacgtcccagac



cc010             Reverse biobricking of {}

ccagtggatcccccagcgtagtctgggacgtcgtatggg 

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K112506/1

Member of these Collections

• //proteindomain/affinity
• //proteindomain/internal/special
• iGEM Parts Registry