BBa_K112601

BBa_K112601 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112601

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sherine Cheung

Date created: 2008-09-02 11:00:00

Date modified: 2015-05-08 01:09:18

< AP tag ! in BBb

Details

• Types: DnaRegion
• Roles: Tag; tag
• Sequences: BBa_K112601_sequence (Version 1)

Description

This is an AP tag with stop codon, but no start.

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This part is in BBb Format. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

BBb Standard Description Page

Notes

N/A

Source

Construction of {
Wobble PCR SC001/SC003 (69 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+ 910)
Product is pBca1256-K112601

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SC001 Forward Biobricking of cgataGAATTCatgAGATCTggcctgaacgatatttttgaagcgcag
SC003 Reverse Biobricking of ccagtGGATCCttattcatgccattcaattttctgcgcttcaaaaatatcg

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K112601/1

Member of these Collections

• //proteindomain/affinity
• //proteindomain/tail
• iGEM Parts Registry