BBa_K112801

BBa_K112801 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K112801
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Anderson
Date created: 2008-05-30 11:00:00
Date modified: 2015-05-08 01:09:19

{mRFP2}{mRFP2} Red Fluorescent Protein with start and Stop



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K112801_sequence (Version 1)

    Description

    This is an mRFP (red fluorescent protein) in BBb format with both start and stop codons.

    ?????????This part is in BBb Format.????????? It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BBb Format is available at:

    http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard

    Notes

    atgatatttgatgctagtttaaagAAGTTGAGGAAATTATTTGTAAATCCAATTGGGTTTTTCCGTGACTCATGGTTTTTTAATTCTAAAAACAAGGCTGAAGAGCTACTATCACCGTTAAAAATAAAAAGTAAAAATATTTTTATAATTAGTAACCTGGGGCAATTAAAAAAAGCTGAGTCATTTGTACAAAAATTTAGCAAGAGAAGTAACTATCTTATTGTTTTGGCAACTGAAAAAAATACTGAGATGCCAAAAATTATTGTTGAACAAATAAATAATAAATTATTTTCTTCATACAAGGTACTATTCATTCCAACTTTCCCAAATGTTTTTTCACTTAAAAAGGTTATATGGTTTTATAACGTATATAATTATTTAGTTTTAAATTCAAAAGCTAAAGATGCTTATTTTATGAGCTATGCGCAACATTATGCAATCTTCGTATATTTGTTCAAAAAAAATAATATAAGATGTTCATTAATTGAAGAGGGGACAGGGACTTATAAAACCGAAAAAGAAAACCCAGTAGTAAATATTAATTTTTATTCAGAGATTATTAATTCAATTATCTTGTTCCATTATCCAGATTTGaaatttgaaaatgtatacggtacatatccaattttgcttaagaaaaaatttaatgcgcaaaaatttgttgagtttaaaggtgctccatcagttaaatcatcaaccagaatagataatgttatccataaatattctataactagagatgatataatatatgcaaatcaaaagtatttgattgaacatacattatttgcggATTCGTTAATTTCTATCTTACTTAGAATAGATAAGCCTGATAATGCAAGAATATTTATAAAACCTCACCCTAAAGAGCCTAAAAAAAATATTAATGCAATTCAAAAGGCAATAAAAAAGGCAAAATGTCGTGACATAATTCTTATAACAGAGCCAGACTTTTTAATAGAGCCGGTAATAAAAAAAGCAAAAATAAAACACTTAATTGGATTAACATCATCTTCTTTGGTATATGCACCTTTAGTTTCTAAAAGATGTCAGTCTTATTCAATAGCGCCTCTTATGATAAAGTTGTGTGATAATGATAAATCCCAAAAAGGGATTAATACGCTGCGTCTCCATTTCGATATTTTAAAGAATTTTGATAATGTTAAAATATTATCGGATGATATAACATCTCCCTCTTTGCACGATAAAAggattttcttgggggagtaa

    Source

    Constructing the pSB1A2 variant (pBca9145) and mRFP basic part (Bca1089)

    PCR ca1089F/R on I13521 (~780 bp, EcoRI/PstI)
    Sub into pSB1A2-I13521 (EcoRI/PstI)
    Product is pBca9145-Bca1089

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K112801/1
     

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