Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: UBC iGEM 2013
Date created: 2013-08-29 11:00:00
Date modified: 2015-05-08 01:09:19
CaMXMT1 under pTET constitutive promoter
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1129014_sequence (Version 1) |
Description
CaMXMT1 is the gene coding for the second enzyme of the caffeine biosynthesis pathway, known as 7-methylxanthosine N-methyltransferase or theobromine synthase, when starting with xanthosine as substrate. TU Munich's 2012 team isolated this gene and added a strep tag at the end, in addition to transferring it all into yeast. We modified it further by removing the yeast consensus sequence and placing the gene and strep tag behind a constitutive promoter and bacterial consensus sequence. This system was transformed into a prokaryote, specifically E. coli strain K12. We have yet to test for expression, and we may find that codon optimization is needed.Notes
Codon optimization has not yet been performed, but we suspect it may be needed for successful expression in a prokaryotic system.Source
The constitutive promoter comes from part BBa_J23118, found in Plate 1 of the 2012 Distribution Kit. The gene comes from TU Munich 2012 part BBa_K801071 in the 2013 Distribution Kit.| Access | Instance | Definition |
| public public public public | BBa_J23118 BBa_B0034 BBa_K801071 BBa_B0015 | BBa_J23118 BBa_B0034 BBa_K801071 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23118 BBa_B0034 BBa_K801071 BBa_B0015 | 1,35 44,55 64,1239 1248,1376 | BBa_J23118 BBa_B0034 BBa_K801071 BBa_B0015 |