BBa_K113007

BBa_K113007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K113007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Wu Jingjing,Wang Jinyu
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:09:20

pBad/araC->T7 polymerase



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K113007_sequence (Version 1)

Description

pBad is a promoter that is tightly controlled by an inducer (L-arabinose) and a repressor (araC). We use it to tightly control the expression of T7 polymerase as in our project the amount of T7 polymerase is essential.

Notes

We used standard assembly to combine pBad/araC and T7 polymerase. The restriction enzymes we used are SpeI and XbaI.

Source

BBa_I0500, iHKU team,

BBa_I712069

Access Instance Definition
public
public
BBa_I0500
BBa_I712069
pBad/araC
BBa_I712069
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K113007/1