Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Toulouse
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:09:21
AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1132037_sequence (Version 1) |
Description
This BioBrick is design to test the AND gate (BBa_K1132034) by measuring the level of RFP after recombination events. The biobrick Bba_K081014 containing the RBS site, the coding sequence of the RFP and a terminator have been inserted inside our gate between the FimE restrictions sites. The T7 polymerase gene is also present in the biobrick, under the control of a strong promoter, strong RBS. This part can therefore be used stand-alone as all elements to control the RFP output are present.Notes
This BioBrick is design to test the AND gate (BBa_K1132034) by measuring the level of RFP after recombination events. The biobrick Bba_K081014 containing the RBS site, the coding sequence of the RFP and a terminator have been inserted inside our gate between the FimE restrictions sites. The T7 polymerase gene is also present in the biobrick, under the control of a strong promoter, strong RBS. This part can therefore be used stand-alone as all elements to control the RFP output are present.Source
synthesis| Access | Instance | Definition |
| public public | BBa_K1132034 BBa_K1132044 | BBa_K1132034 BBa_K1132044 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1132034 BBa_K1132044 | 1,1126 1135,3849 | BBa_K1132034 BBa_K1132044 |