Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Deyell
Date created: 2013-10-03 11:00:00
Date modified: 2015-05-08 01:09:21
pSB9S1
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K1137003_sequence (Version 1) |
Description
Contains the mRFP cassette (J04450) and additionally terminators and flanking region from pSB1C3. The origin of replication has been replaced with CloDF13 replicon with a copy number of 20 ??? 40 per cell. The cat gene has been replaced with aadA conveying spectinomycin and streptomycin resistance.Notes
The terminators in the pSB1C3 made gibson assembly difficult and as such we switched to traditional cloning using XhoI and AvrII cut sites.Source
Upgraded pSB1C3 swapping out the origin and resistance cassettes for CloDF13 and aadA from pCDF| Sequence Annotation | Location | Component / Role(s) |
| aadA CloDF13 ori terminator terminator terminator mRFP prefix stem loop terminator suffix | 122,910 1050,1788 1705,1726 1762,1784 1887,1927 2177,2854 1929,1949 2986,3005 3041,3110 3021,3040 | feature/protein CDS sequence_feature feature/dna feature/stem_loop stem_loop stem_loop feature/stem_loop stem_loop feature/stem_loop CDS feature/cds engineered_region feature/BioBrick feature/stem_loop stem_loop feature/stem_loop stem_loop feature/BioBrick engineered_region |