BBa_K1140001

BBa_K1140001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1140001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Janssel Reyes del Castillo

Date created: 2013-09-07 11:00:00

Date modified: 2015-05-08 01:09:22

pTet + 37 oC Riboswitch + RFP (LVA)

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1140001_sequence (Version 1)

Description

A 37??C RiboSwitch that regulate the transcription of red fluorescent protein (with LVA tag). The part includes the pTet promoter.

Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.

Source

Obtained synthetically.
Promoter pTet.
Based on a fusion of a RBS and a Riboswitch.
RFP.
(Further information coming soon)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pTet	1,54	promoter, feature/promoter
37 oC riboswitch	63,121	stem_loop, feature/stem_loop
RFP (LVA)	122,835	feature/cds, CDS
T7 double terminator	844,887	feature/stop, stop_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1140001/1

Member of these Collections

• iGEM Parts Registry