BBa_K1140002

BBa_K1140002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1140002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Janssel Reyes del Castillo

Date created: 2013-09-07 11:00:00

Date modified: 2015-05-08 01:09:23

pLac + 32 oC RNA thermometer + GFP(LVA)

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1140002_sequence (Version 1)

Description

A Green fluorescent protein (with LVA tag) that is regulated by a 32 degrees riboswitch. The part includes a the promoter "pLac".

Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.

Source

Obtained synthetically. Promoter pLac.
Based on a fusion of a RBS and a Riboswitch. GFP (LVA). T7 double terminator(Further information coming soon).

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pLac	1,56	promoter, feature/promoter
RNA thermometer	65,147	feature/stem_loop, stem_loop
GFP+LVA	154,912	CDS, feature/cds
T7 terminator	920,964	stem_loop, feature/stem_loop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1140002/1

Member of these Collections

• iGEM Parts Registry