Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: C??cile Qu??r??
Date created: 2013-10-11 11:00:00
Date modified: 2015-05-08 01:09:26
4CL::STS (resveratrol synthase)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1148013_sequence (Version 1) |
Description
This part should allow resvertrol biosynthesis by the coupling of p-coumaric acid to CoA by the 4CL enzyme. Subsequently, coumaroyl-Co1 is converted into resveratrol by STS thanks to sequential addition of three malonyl-CoA units with the release of carbon dioxide.It is under the control of BBa_R0011 a lactose inducible promoter.
Notes
Starting from eucaryote DNA was a challenge to get a sequence without introns.Source
The promoter was taken from the registry. 4CL and STS were amplified from grape cDNA.| Access | Instance | Definition |
| public public | BBa_R0011 BBa_K1148012 | lacI+pL BBa_K1148012 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0011 BBa_K1148012 | 1,54 62,2845 | lacI+pL BBa_K1148012 |