BBa_K115004

BBa_K115004 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K115004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bastiaan van den Berg

Date created: 2008-07-15 11:00:00

Date modified: 2015-05-08 01:09:27

A temperature sensitive 5

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_K115004_sequence (Version 1)

Description

This part is designed as a temperature inducible RBS. Only designed so far.

Notes

The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.

Source

The sequence is based on the paper "RNA Quadruplex-Based Modulation of Gene
Expression" by Markus Wieland and Jorg S. Hartig.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SD	22,28	ribosome_entry_site, feature/rbs
start	36,38	feature/start, start_codon
His-Tag	42,71	feature/tag, tag

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K115004/1

Member of these Collections

• iGEM Parts Registry