Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tania Christiansen
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:29
TycA_deltaCom Module from Tyrocidine
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1152000_sequence (Version 1) |
Description
This is the CDS for TycA_deltaCom module from the Tyrocidine gene cluster of Brevibacillus Parabrevis.It is the first module in this cluster for the NonRibosomalPeptide-Synthetase of the cyclic decamer Tyrocidine.
This specific module Tyca_deltaCom is responsiple for the binding of the first amino acid D-Phenyl of the NRP.
It consists of the A- (adenylation), PCP- (peptide carrier protein) and the E- (epimerization) domain. After Tyc A follows the TycB gene with three modules.
This bio brick can be used for the design of different NRPS.
It was used in the synthetic Tripeptide I of our project.
Notes
This is just the coding sequence. We sequenced with VR and VF2 primer, not mutations were found.Source
Tyrocidine Gene Cluster of Brevibacillus Parabrevis. Genebank File taken from http://www.ncbi.nlm.nih.gov/nuccore/af004835| Sequence Annotation | Location | Component / Role(s) |
| Coding Sequence TycA_deltaCom ATG Start Codon | 1,3186 1,3 | CDS feature/cds feature/start start_codon |