BBa_K1159212

BBa_K1159212 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1159212
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dong-Jiunn Jeffery TRUONG
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:31

Membrane-anchored SpyCatcher with secretory SpyTag_nLuc reporter fusion translational unit



Types
DnaRegion

Roles
engineered_region

Translational_Unit

Sequences BBa_K1159212_sequence (Version 1)

Description

This part encodes a translational unit consisting two fusion proteins, a Membrane-anchored SpyCatcher and a secretory SpyTag_nLuc (IgKappa-SigP_SpyTag_nLuc) reporter fusion to show if post-translational protein fusion like reaction happens between the membrane-localized catcher protein and the secretory fusion protein containing N-terminal SpyTag and C-terminal NanoLuc Luciferase. As a translational unit both fusion proteins are seperated by a polioviral IRES to allow bicistronic expression in eukaryotic systems. To express this system you only have to insert your favored promoter/terminator in the prefix/suffix of this part. For further information, please see under the subparts linked in the text.

Notes

x

Source

Synthetic

igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1159212/1