Vio A

BBa_K1161000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1161000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Esau
Date created: 2013-09-11 11:00:00
Date modified: 2015-05-08 01:09:32

violacein A coding sequence



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1161000_sequence (Version 1)

Description

Violacein A (Vio)A is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour.

Notes

ORf is identical to its BBa_K274002 equivalent except for the modification noted above

Source

Source: BBa_K274002 2010 registery of biological parts

Sequence Annotation Location Component / Role(s)
B0030
Spe/Pst
cds
1,15
16,21
22,1278
feature/rbs ribosome_entry_site
feature/misc sequence_feature
CDS feature/cds
igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1161000/1