Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kathleen Miller
Date created: 2013-09-14 11:00:00
Date modified: 2015-05-08 01:09:32
10x-Histidine (10x-His) Tag with Met (ATG) For N-terminal purification
| Types | DnaRegion |
| Roles | tag Tag |
| Sequences | BBa_K1162009_sequence (Version 1) |
Description
This part uses assembly standard 23 (RFC 23-silver fusion) to allow for protein fusions. This 10x His Tag is similar to BBa_K844000 but has the double stop codon (TAA TAA) removed unlike BBa_K844000. (http://parts.igem.org/wiki/index.php?title=Part:BBa_K844000).Another characteristic of this part is the presence of a Met (ATG) for direct His Tag and protein expression. Since the presence of the Met (ATG) this His Tag should be used for protein purification at the N-terminal. The user can place a promoter-rbs system of their choice in front of this His Tag for direct expression. The lack of any stop codon in this His Tag allows the user to add additional features downstream of the gene e.g. secretion tag or GFP (note, the gene itself must not contain any stop codons if the His Tag is to be used like this).
Related parts
BBa_K844000 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K844000), this link also provides an extensive review of existing Histidine Tags in the Parts Registry.