BBa_K1166003

BBa_K1166003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1166003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Luis Mario Leal Garza

Date created: 2013-09-16 11:00:00

Date modified: 2015-05-08 01:09:33

His-TAT

Details

• Types: DnaRegion
• Roles: engineered_region; Signalling
• Sequences: BBa_K1166003_sequence (Version 1)

Description

A biobrick that includes the TAT signal peptide that can be fused to any protein for an easy internalization into mammal cells. It includes a 6x His-tag for easy purification.
TAT is a cell penetrating peptide (CPP) derived from the transactivator of transcription from HIV and has been used to internalize a range of large and small molecules from peptides, DNA and antibodies, to liposomes.

Notes

We include a 6x Histidine tag for purification. TAT peptide is ready to be fused at N-terminal region.

Source

We took the sequences needed for these part from a sequence alignment.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
start	1,3	feature/start, start_codon
His-tag	4,21	feature/tag, tag
linker	22,51	sequence_feature, feature/misc
TAT	52,84	CDS, feature/cds
linker	85,114	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1166003/1

Member of these Collections

• iGEM Parts Registry