CIIICd

BBa_K116609 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K116609

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jesse Wu

Date created: 2008-10-29 12:00:00

Date modified: 2015-05-08 01:09:33

CIIICd: modified CIII coding region from λ
phage

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K116609_sequence (Version 1)

Description

CIIICd: A modified version of the CIII coding region from λ phage.
Original CIII:
ATGCAATATGCCATTGCAGGGTGGCCTGTTGCTGGCTGCCCTTCCGAATCTTTACTTGAACGAATCACCC
GTAAATTACGTGACGGATGGAAACGCCTTATCGACATACTTAATCAGCCAGGAGTCCCAAAGAATGGATC
AAACACTTATGGCTATCCAGACTAA
The protein domains from the left-most and right-most side of CIII have been removed (the grayed out parts), i.e. amino acid residues #2-13 and #42-49 have been excluded, leaving residues #1 (start codon), #14-41 (the protein domain in the middle) and #55 (stop codon).

Notes

HtlB also degrades CIII, creating competitive degradation reactions. We removed the leftmost and right most protein domains since Hadler et al said that removing these two protein domains will stop HtlB from degrading CIII. Doing this makes it easier to predict and model.

Source

λ phage

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K116609/1

Member of these Collections

• //cds/transcriptionalregulator
• iGEM Parts Registry