Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas K. Goldner
Date created: 2013-09-16 11:00:00
Date modified: 2015-05-08 01:09:35
beta-galacturonidase (YesZ) from Bacillus Subtilis 168
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1175007_sequence (Version 1) |
Description
yesZThe enzyme acts on the terminal end of side chains of Rhamnogalacturonan I Pectin, releasing the free galactose. This gene has been isolated from the bacterium Bacillus subtilis subtilis 168.
The enzyme yesZ has beta-galactosidase (beta-galacturonidase) activity, cleaving (1→4)-β-D-galactans to produce single galactose molecules from RG I Pectin.
Usage and Biology
Pectin is a component of the primary cell wall of plants. As galactose can be metabolized by the human body and by animals, it is an alternative to the usual target of nutrition/energy boosts in biotechnology, glucose.
(1) http://www.ncbi.nlm.nih.gov/pubmed/17449691
(2) http://subtiwiki.uni-goettingen.de/wiki/index.php/YesZ
(3) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1694227/
(4) http://aem.asm.org/content/73/12/3803
(5) http://www.tandfonline.com/doi/full/10.1080/15583724.2011.615962#tabModule
Notes
No design considerations were needed, although we did add a second stop codon.Source
Bacillus Subtilis 168 genomic DNA| Sequence Annotation | Location | Component / Role(s) |
| Start Codon YesZ Enzyme Double Stop Codon | 1,3 4,1988 1989,1995 | feature/start start_codon CDS feature/protein feature/stop stop_codon |