| Types | DnaRegion
|
| Roles | Reporter
engineered_region
|
| Sequences | BBa_K1204013_sequence (Version 1)
|
Description
This is a strong constitutive promoter in yeast with a binding site downstream. The binding site is designed to be directly targeted by our iTAL 16A or gRNA 16A. When the binding site is occupied by an iTAL or gRNA/dCas9 complex, the expression of the YFP reporter should be knocked down.
Notes
We had to consider the manner in which iTALs and gRNA/dCas9 complex bind in designing our sequence.
Source
The binding site was synthetically designed using the binding rules for iTALs and gRNA/dCas9 complex and ordered as a gene block from IDT. The TEF1 promoter is a strong constitutive promoter in yeast that we got from another plasmid in the lab.
