BBa_K1211001

BBa_K1211001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1211001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joel Sher
Date created: 2013-08-15 11:00:00
Date modified: 2015-05-08 01:09:41

Clostridum propionicum propionate CoA transferase



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1211001_sequence (Version 1)

Description

This is the DNA coding for the enzyme propionate CoA transferase from Clostridum propionicum. The enzyme normally catalyzes the reaction: acetyl-CoA + propanoate <--> acetate + propanoyl-CoA. This mutated sequence has four point mutations and one amino acid substitution (compared to the wild type enzyme) in order to produce (D)-Lactyl-CoA.

Notes

The chose to introduce these mutations based upon Yang et al. 2009

Source

The sequence of the wild type enzyme came from GenBank.

Sequence Annotation Location Component / Role(s)
PctCp
1,1575
engineered_region feature/BioBrick
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1211001/1