BBa_K1212001

BBa_K1212001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1212001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Aura Ferreiro

Date created: 2013-09-15 11:00:00

Date modified: 2015-05-08 01:09:41

Theophylline Riboswitch (Clone E)

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_K1212001_sequence (Version 1)

Description

Encodes a RNA secondary structure that in the presence of theophylline undergoes a conformational change that makes available the previously sequestered RBS, thus allowing transcription of the downstream sequence. Achieves a 60-fold activation ratio in 2mM theophylline as reported by Topp et al. in: 'Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species.' Appl. Environ. Microbiol. December 2010 vol. 76 no. 23 7881-7884

Notes

None.

Source

Appl. Environ. Microbiol. December 2010 vol. 76 no. 23 7881-7884. Synthetic riboswitch generated through rational design and in vivo screening.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Theophylline aptamer	1,38	feature/stem_loop, stem_loop
RBS	44,51	feature/rbs, ribosome_entry_site

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1212001/1

Member of these Collections

• iGEM Parts Registry