| Types | DnaRegion
|
| Roles | promoter
Regulatory
|
| Sequences | BBa_K1225000_sequence (Version 1)
|
Description
This is a medium-strength Escherichia coli Ptrc* promoter. The asterisk denotes the lack of an operator downstream of the transcription start site, -35 to +1 of the promoter. The promoter's sequence comes from "Precise and reliable gene expression via standard transcription and translation initiation elements" by Vivek K. Mutalik and colleagues. Two inward pointing BpiI (BbsI) sites were added between the original promoter sequence and the BioBrick prefix and suffix to allow seamless assembly into the 2013 Purdue iGEM team's Bicistronic Design (BCD) Expression Operating Units, modified versions of BCDs 7, 18, 22 and 23 created by Mutalik et al. in the aforementioned work.
Notes
The BpiI binding sites lie between the promoter and BioBrick cut sites, so the part can be handled at HQ but can also be seamlessly cloned into our BCD EOUs with Golden Gate assembly.
Source
The Ptrc promoter comes from E. coli.
