BBa_K1230015

BBa_K1230015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1230015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Andr??s Ferri??o Iriarte

Date created: 2013-09-25 11:00:00

Date modified: 2015-05-08 01:09:44

araC-pBad-rbs-LL37-rbs-GFP-pLacI-rbs-MarA Generator

Details

• Types: DnaRegion
• Roles: Project; engineered_region
• Sequences: BBa_K1230015_sequence (Version 1)

Description

LL-37 antimicrobial peptide with human codon usage under the Arabinose promoter with GFP generator. The MarA protein acts as a transcriptional activator in E. coli. It allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to cationic antimicrobial peptides, such as LL-37. This composite part allows MarA expression in presence of IPTG, by using the LacI promoter and the RBS based on Elowitz repressilator.

Notes

LL-37 has the codon usage for humans. Using E. coli codon usage may inhibit protein function by wrong folding.

Source

Genscript
2013 DNA distribution

Components

Access	Instance	Definition
public	BBa_K1230011	BBa_K1230011
public	BBa_K1230014	BBa_K1230014

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1230011	1,1355	BBa_K1230011
BBa_K1230014	1364,2868	BBa_K1230014

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1230015/1

Member of these Collections

• iGEM Parts Registry