BBa_K1300000

BBa_K1300000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1300000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: StuyGem
Date created: 2014-06-15 11:00:00
Date modified: 2015-05-08 01:09:46

recA promoter + Key (taRNA)



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1300000_sequence (Version 1)

Description

RecA is a UV-light-inducible promoter. Trans is a "key" of sorts, acting on a complementary cis "lock" to allow the translation of ccdB, a kill gene. Together, the RecA+Trans system allows for the kill gene to be translated in the presence of UV light, through the indirect induction by way of the cis-trans system.

Notes

We had to consider whether the design was too complicated, whether it wouldn't be better to just have a more simple, direct system whereby a kill gene was translated because of the action of a UV-light-inducible promoter. In the end, we decided to make both systems, as the latter has been done before and submitted to the iGEM Registry of Standard Biological Parts.

Source

Source parts were obtained from the iGEM Registry of Standard Biological Parts.

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1300000/1