NeoR

BBa_K1313004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1313004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Carlos Meza

Date created: 2014-10-11 11:00:00

Date modified: 2016-01-28 01:43:08

Neomycin Resistance

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1313004_sequence (Version 1)

Description

Neomycin resistance gene. This gene can be used for selection of cells with an efficient transformation. This gene codes neomycin phosphotransferase which breaks neomycin, kanamycin, and gentamicin molecules. If you use these gene as selectivity gene in bacteria, and your laboratory lacks neomycin, you can replace it for kanamycin by adding 35 mg/ml per liter of LB media. The best concentration of neomycin to use is 80 mg/ml per liter of LB media.


This gene can be also used as a selective gene for mammalian cells using gentamicin.

Notes

Pst1 site cuts gene.

Source

This gene was obtained from pCDNA 3.1 myc His(A).

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1313004/1

Member of these Collections

• //cds/selectionmarker/antibioticresistance
• iGEM 2018 Distribution Plate 5
• iGEM 2018 Distribution Plate 5 Well 24D
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry