BBa_K1315000

BBa_K1315000 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K1315000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dimitrios Michailidis

Date created: 2014-09-19 11:00:00

Date modified: 2015-05-08 01:09:48

pqsR

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1315000_sequence (Version 1)

Description

The Pseydomonas quinolone signal (PQS) as well as its precursor 2-heptyl-4-quinolone (HHQ)are very important in mechanisms controlling virulence in Pseudomonas aeruginosa. P. aeruginosa has a key role in Cystic Fibrosis (CF). PQS can initiate signalling for various processes like virulence, motility, and biofilm formation. A good number of these processes are facilitated by PqsR, which is a transcriptional factor. An activated PqsR will go to the nucleus of the cells and activate the respective genes.

http://www.ncbi.nlm.nih.gov/pubmed/21539583
http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs
http://www.ncbi.nlm.nih.gov/pubmed/12393198

Notes

The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.

Source

The source of this part is the genome of Pseudomonas aeruginosa PAO1 provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
PqsR	1,999	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1315000/1

Member of these Collections

• //cds/transcriptionalregulator/activator
• //chassis/prokaryote/ecoli
• //direction/forward
• //function/regulation/transcriptional
• //regulation/positive
• iGEM Parts Registry