Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yanwen Xie
Date created: 2014-09-22 11:00:00
Date modified: 2015-05-08 01:09:58
lambda cI-LGF2(R74A)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1333202_sequence (Version 1) |
Description
Fuse lambda cI with LGF2 mutated from Arg to Ala at site 74 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes a stronger interaction with GAL11P compared to the normalized WT.Notes
The LGF2 is mutated from CGA to GCA at site 74.Source
It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon TGA stop codon lambda cI LGF2(R74A) | 1,3 841,843 1,711 721,840 | feature/start start_codon feature/stop stop_codon CDS feature/protein CDS feature/protein |