Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yanwen Xie
Date created: 2014-09-22 11:00:00
Date modified: 2015-05-08 01:09:58
lambda cI-LGF2(D76A&D78A)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1333203_sequence (Version 1) |
Description
Fuse lambda cI with LGF2 mutated from Asp to Ala at sites 76 and 78 (the Glu which locates after three continuous Ala behind lambda repressor is at site 58). As one of the components in Bacterial Two-hybrid System, it causes little interaction with GAL11P compared to the normalized WT.Notes
The LGF2 is mutated from GAC to GCC at site 76 and from GAC to GCC at site 78.Source
It is from Agilent Technologies' BacterioMatch ⅡTwo-Hybrid System Vector Kit and modified by Site-Directed Mutagenesis(SDM).| Sequence Annotation | Location | Component / Role(s) |
| ATG start codon TGA stop codon lambda cI LGF2(D76A&D78A) | 1,3 841,843 1,711 721,840 | start_codon feature/start stop_codon feature/stop CDS feature/protein feature/protein CDS |