BBa_K1334017

BBa_K1334017 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1334017
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Qiushuang Wu
Date created: 2014-09-26 11:00:00
Date modified: 2015-05-08 01:09:58

PADH from Pseudomonas putida



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K1334017_sequence (Version 1)

    Description

    PADH from Pseudomonas putida is a formaldehyde dehydrogenase which can oxidize free formaldehyde to formate independent of GSH.This part is the coding sequence for the enzyme PADH

    Notes

    The original sequence contains a Not1 site,by point mutation,we removed the site

    Source

    Using the genomic DNA of P. putida as the template, the coding regions of the PADH gene (padh) was amplified by PCR. The PCR products were then subcloned into PSB1C3

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    Planning
     
    synbiohub#ownedBy
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    synbiohub#ownedBy
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    synbiohub#topLevel
    BBa_K1334017/1
     

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