BBa_K1343010

BBa_K1343010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1343010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon

Date created: 2014-09-26 11:00:00

Date modified: 2015-05-08 01:09:59

PCI-lacI

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1343010_sequence (Version 1)

Description

LacI repressor protein under Lambda phage repressor CI regulated promoter

Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other

Source

Placeholder

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
promoter lamda CI regulator	1,49	feature/promoter, promoter
barcode label 6	50,71	sequence_feature, feature/misc
barcode label 1	72,93	sequence_feature, feature/misc
barcode label 4	94,113	sequence_feature, feature/misc
RBS	114,128	sequence_feature, feature/misc
space	129,134	sequence_feature, feature/misc
lacI	135,1226	CDS, feature/protein
barcode label 3	1227,1248	feature/misc, sequence_feature
barcode label 2	1249,1270	feature/misc, sequence_feature
barcode label 5	1271,1292	feature/misc, sequence_feature
Terminator	1293,1421	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1343010/1

Member of these Collections

• iGEM Parts Registry