BBa_K1343012

BBa_K1343012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1343012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
Date created: 2014-09-26 11:00:00
Date modified: 2015-05-08 01:09:59

Plux-amilCP



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K1343012_sequence (Version 1)

Description

blue reporter protein amilCP under LuxR-AHL regulated promoter

Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other

Source

Placeholder

Sequence Annotation Location Component / Role(s)
Barcode label 3
BBa_R0062: Plux
Barcode label 6
Barcode label 1
BBa_B0030: Strong RBS
BBa_K592009: amilCP
Barcode label 4
Barcode label 3
Barcode label 2
Barcode label 5
BBa_B0010: T1 (terminator) from E. coli
T7 terminator
100,121
1,55
56,77
78,99
122,136
143,808
809,830
831,852
853,874
875,896
897,976
977,1023
feature/misc sequence_feature
feature/promoter promoter
feature/misc sequence_feature
feature/misc sequence_feature
ribosome_entry_site feature/rbs
CDS feature/protein
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
feature/BioBrick engineered_region
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1343012/1