BBa_K1343013

BBa_K1343013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1343013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
Date created: 2014-09-26 11:00:00
Date modified: 2015-05-08 01:09:59

PT7-amilCP



Types
DnaRegion

Roles
engineered_region

Reporter

Sequences BBa_K1343013_sequence (Version 1)

Description

blue reporter protein amilCP under T7 promoter

Notes

To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each other

Source

Placeholder

Sequence Annotation Location Component / Role(s)
T7 Promoter
Barcode label 6
Barcode label 1
Barcode label 4
BBa_B0030: Strong RBS
BBa_K592009: amilCP
Barcode label 3
Barcode label 2
Barcode label 5
BBa_B0010: T1 (terminator) from E. coli
T7 terminator
1,23
24,45
46,67
68,89
90,104
111,776
777,798
799,820
821,842
843,922
923,969
promoter feature/promoter
sequence_feature feature/misc
sequence_feature feature/misc
feature/misc sequence_feature
feature/rbs ribosome_entry_site
feature/protein CDS
feature/misc sequence_feature
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1343013/1