Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tal Naor, Ricarina Rabinovitz, Ittai Rubinstein, Yair Maimon
Date created: 2014-09-26 11:00:00
Date modified: 2015-05-08 01:09:59
Plux-green renilla luciferase
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K1343014_sequence (Version 1) |
Description
E.coli optimized green renilla luciferase under LuxR-AHL regulated promoterNotes
To assamble this part, and others like it, we used a specialized Gibson Barcode System to allow for a modular construction of gates with various components using the same Gibson reaction conditions, allowing for the assembly of a large number of constructs in parallel to each otherSource
Placeholder| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0062: P lux barcode 6 barcode 1 barcode 3 BBa_B0030: Strong RBS green renilla luciferase barcode 4 barcode 2 barcode 5 BBa_B0010:T1 (terminator) from E. coli T7 terminator | 1,55 56,77 78,99 100,121 122,136 143,1078 1079,1100 1101,1122 1123,1144 1145,1224 1225,1271 | promoter feature/promoter feature/barcode engineered_tag feature/barcode engineered_tag engineered_tag feature/barcode ribosome_entry_site feature/rbs feature/protein CDS engineered_tag feature/barcode feature/barcode engineered_tag feature/barcode engineered_tag feature/stop stop_codon feature/stop stop_codon |