BBa_K1351026

BBa_K1351026 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1351026

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Mona Dotzler

Date created: 2014-10-04 11:00:00

Date modified: 2015-06-01 12:56:16

Signal peptide of B. subtilis major autolysin LytC

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1351026_sequence (Version 1)

Description

Signal peptide (amino acids 1 to 25) of Bacillus subtilis major autolysin LytC (N-acetylmuramoyl-L-alanine amidase, Uniprot Q02114). Targets proteins for secretion via the Sec pathway (Tjalsma et. al., 2000).

Notes

none

Source

This part was generated by amplification from BioBrick BBa_K1351006 with the primers listed below, followed by digestion with EcoRI and PstI and ligation into pSB1C3.

LytC_ENX_SD_Ngo_fwd: gatcGAATTCgcggccgctTCTAGAgtaaggaggaaGCCGGC TTGCGTTCTTATATAAAAGTCCTAACAATGTG

LytcSP_SNP_Age_rev: gatcCTGCAGcggccgctACTAGTattaACCGGT ATCGGCCAAAGCTGTTGGC

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1351026/1

Member of these Collections

• //cds
• //chassis/prokaryote/bsubtilis
• iGEM 2018 Distribution Plate 5
• iGEM 2018 Distribution Plate 5 Well 4D
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry