BBa_K1352010

BBa_K1352010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1352010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ana-Maria Cujba, Martyna Sroka
Date created: 2014-09-30 11:00:00
Date modified: 2015-05-08 01:10:02

Ag43 autotransporter (ORF)



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K1352010_sequence (Version 1)

    Description

    Open reading frame of Ag43 autotransporter protein engineered to comply with RFC10

    Notes

    6 additional PstI restriction sites ( C_TGCA^G) within the open reading frame of Ag43 from BioBrick BBa_K759001 have been removed through the insertion of synonymous mutations (CTGCAA). These were introduced through PCR-based Site-Directed Mutagenesis (Agilent Lightning Quik-Change mutagenesis kit)

    Source

    PCR amplified from BioBrick BBa_K759001, then subjected to Site-Directed Mutagenesis.

    Sequence Annotation Location Component / Role(s)
    Ag43
    		1,3120
    		feature/cds CDS

    igem#experience
    Works
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1352010/1
     

    Attachments

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