Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Luna Barroco de Lacerda, Maria Clara Tavares Astolfi
Date created: 2014-10-05 11:00:00
Date modified: 2015-05-08 01:10:02
Mercury ions bioremediating device
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1355004_sequence (Version 1) |
Description
The biorremediator device is composed by our ???essential biobrick??? (BBa_K1355001) + a MerA gene (BBa_K1355000). In the presence of mercury, The MerR protein will bind to it and MerT, MerP and MerA protein will be expressed and bioremediation will start! The MerP and MerT proteins are responsible for the transport of mercury from the periplasm to the cytoplasm, leading him to the mercury ion reductase (MerA)! The beautiful MerA be responsible for the reduction from Hg2+ in Hg0, which is volatile at room temperature and able to passively leave out the bacteria.Notes
For this construct, we followed these steps: Extraction of plasmid DNA and DNA quantification of bacteria transformed with the Essential Biobrick (BBa_K1355001) and the bacteria transformed with the MerA Biobrick (BBa_E0840); Verifying the electrophoretic profile of the extracted plasmid DNA; Restriction enzyme digestion for Essential Biobrick with SpeI and PstI and for merA Biobrick with PstI and XbaI; Checking the electrophoretic profile of digested samples; Fragment of interest purification from the gel; Ligation the gel purification of merA gene and digested Essential Biobrick; Transformation of ligation in DH5-alpha; Plasmid DNA extraction from bacteria transformed; Check the electrophoretic profile to see results of samples linked (no fragments); Digestion of Essential Biobrick + merA Biobrick with EcoRI and PstI, aiming to analyze the fragment size to be isolated; Checking the electrophoretic profile of the digested sample to obtain results showing that the isolated fragment is the junction of Essential Biobrick +merA Biobrick ;Source
BBa_K1355001, BBa_K1355000| Access | Instance | Definition |
| public public | BBa_K1355001 BBa_K1355000 | BBa_K1355001 BBa_K1355000 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1355001 BBa_K1355000 | 1,1189 1198,2975 | BBa_K1355001 BBa_K1355000 |