BBa_K136007

BBa_K136007 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K136007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kok-Phen YAN

Date created: 2008-10-24 11:00:00

Date modified: 2015-05-08 01:10:02

flgB promotor (RBS WT)

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K136007_sequence (Version 1)

Description

Promoter of flgB gene from E. coli flagella

flgB is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).

Its promoter is consitutively repressed.

It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon ).

Notes

>forward primer
GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG

>reverse primer
GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT

Source

This part comes from the genome of E.coli K12 strain MG1655.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
flhDC binding site	98,113	non_covalent_binding_site, feature/binding
flhDC binding site	124,139	feature/binding, non_covalent_binding_site
atg	198,200	feature/start, start_codon
transcription initiation site	173,173	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K136007/1

Member of these Collections

• iGEM Parts Registry