Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: alexandra bouaziz
Date created: 2008-10-24 11:00:00
Date modified: 2015-05-08 01:10:03
flgA promoter - YFP Tripart (LVA-)
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K136013_sequence (Version 1) |
Description
Promoter of flgA gene from E. coli flagellaflgA is a class 2 gene that is part of the construction of the flagella ( Ordering Genes in a Flagella Pathway by Analysis of Expression Kinetics from Living Bacteria - S. Kalir, J. McClure, K. Pabbaraju, C. Southward, M. Ronen, S. Leibler, M. G. Surette, U. Alon ).
Its promoter is consitutively repressed.
It is activated by FlhD4C2 and maybe by FliA with different strength ( Using a Quantitative Blueprint to Reprogram the Dynamics of the Flagella Gene Network - Shiraz Kalir and Uri Alon .
flgA promotor is here followed by YFP tripart
Notes
flgA was digested by EcoRI and SpeI (insert) and cloned into the J61002 plasmid psb1A2 containing YFP tripart LVA.Source
flgA promotor comes from E. coli K12 strain MG1655.yfp comes from the registry.
| Access | Instance | Definition |
| public public public public public | BBa_K136006 BBa_B0034 BBa_E0030 BBa_B0010 BBa_B0012 | BBa_K136006 BBa_B0034 eyfp BBa_B0010 BBa_B0012 |