BBa_K1361994

BBa_K1361994 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1361994

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shoujie Sun

Date created: 2014-10-05 11:00:00

Date modified: 2015-05-08 01:10:04

Curli Fiber major monomer-CsgA with his tag

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K1361994_sequence (Version 1)

Description

This part is CsgA(BBa_K1361999) with twin 7Xhis tag. And can be used for purified of CsgA monomer or absorbing Au-NTA-Ni nano partical.
CsgA is the major monomer of curli fiber. In vivo, its alone has little polymerizability and CsgB protein is requested for quick polymerization of CsgA.

Notes

The modifying of CsgA was referring the supplementary of Lu, Michelle Y., et al. "Synthesis and patterning of tunable multiscale materials with engineered cells." (2014).

Source

CsgA-his was synthesized by GeneScrip.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cds	1,498	CDS, feature/cds
7XHis	127,147	feature/tag, tag
7XHis	475,495	feature/tag, tag

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1361994/1

Member of these Collections

• iGEM Parts Registry