BBa_K1362092

BBa_K1362092 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1362092
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:04

pSB1C30: High copy BioBrick cloning/expression backbone carrying Cm resitance



Types
DnaRegion

Roles
plasmid_vector

Plasmid_Backbone

Sequences BBa_K1362092_sequence (Version 1)

Notes

Was archived by cloning an oligo containing the promoter into the EcoRI site of pSB1C3

Sequence Annotation Location Component / Role(s)
suffix
E. coli his operon terminator
terminator stem loop
VR
rep(pMB1) (rev)
ORI
Chloramphenicol resistance
VF2
end of terminator
terminator stem loop
T7 promotor
lacO reg
prefix
T0 terminator
1,21
22,93
30,64
157,176
261,875
276,276
1160,1819
1933,1952
2009,2012
2022,2041
2054,2072
2072,2099
2100,2121
1042,1147
engineered_region feature/BioBrick
sequence_feature feature/misc
feature/stem_loop stem_loop
primer_binding_site feature/primer_binding
sequence_feature feature/misc
sequence_feature feature/misc
feature/cds CDS
primer_binding_site feature/primer_binding
sequence_feature feature/misc
stem_loop feature/stem_loop
feature/promoter promoter
feature/operator operator
engineered_region feature/BioBrick
stem_loop feature/stem_loop
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1362092/1