RFC[105] E

BBa_K1362420 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1362420
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena B??scher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Sch??fer, Carolin Schmelas, Silvan Schmitz, Max Waldha
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:10:05

C-terminal non-splicing overhang AGGC=1/3Ala+Gly RFC[105] E



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_K1362420_sequence (Version 1)

Description

This is a standard overhang sequence for in-frame cloning of Proteins of Interest in front or behind an Intein. A detailed cloning strategy is found on the iGEM team Heidelberg 2014's wiki page as well as in RFC[???]. Specifically, this part contains the overhang E* used to insert a protein in front of a non-splicing N-Intein, which could often serve as control for protein splicing experiments. It lies within the four bases formed by the glycine and the first base of the subsequent Leucine/Isoleucine or similar of the mutated N-terminal splicing site.

Notes

This part is only the sequence of a standard overhang. It will not be sent in as physical DNA and was merely created to easily compose new parts in the RFC[???] standard. In the actual cloning process this sequence was and is recommended to be inserted with the according PCR primers.

Source

This standard overhang part was developed as part of the iGEM team Heidelberg 2014's Intein Toolbox [[#References|[1]]]. All standard sequences can be reviewed in RFC[???] [[#References|[2]]].

igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1362420/1