<- BsaI

BBa_K1362427 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1362427
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Constantin Ahlmann-Eltze, Charlotte Bunne, Magdalena Büscher, Jan Gleixner, Max Horn, Anna Huhn, Nils Klughammer, Jakob Kreft, Elisabeth Schäfer, Carolin Schmelas, Silvan Schmitz, Max Waldha
Date created: 2014-10-07 11:00:00
Date modified: 2015-05-08 01:10:05

BsaI reverse restriction site for RFC[???] cloning (shortened by one C)



    • Details

    Types
    DnaRegion

    Roles
    sequence_feature

    DNA

    Sequences BBa_K1362427_sequence (Version 1)

    Description

    This Sequence starts with a part of the reverse-complemented BsaI recognition site. The missing C of the recognition site must be included in the downstream part. It contains a spacer thymidine so that BsaI will cut the bottom strand directly upstream and the top strand 4 nucleotides upstream.

    Source

    BsaI recognition sequence from rebase

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1362427/1
     

    Attachments

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