BBa_K1364013

BBa_K1364013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1364013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aur??lie Kanitzer
Date created: 2014-09-30 11:00:00
Date modified: 2015-06-08 08:36:54

Pveg - RBS - Antifungal GAFP-1 - RBS - Antifungal D4E1 - Double terminator



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K1364013_sequence (Version 1)

Description

The Gastrodia anti-fungal protein (GAFP-1) is a mannose-binding lectin which is able to inhibit the growth of multiples species of plant pathogenic fungi.

D4E1 is a linear synthetic peptide of 17 amino acids which has shown to have antifungal activities by complexing with a sterol present in conidia's wall of a variety of fungi.

The RBS used is a strong RBS for Bacillus subtilis (K780002).

Notes

This part is designed for the co-expression of two different peptides with anti-fungal activities : D4E1 and GAFP-1. It is composed of the strong, constitutive promotor of B. subtilis Pveg (K823003) and the translation unit with GAFP-1 and D4E1 (BBa_K1364012).

http://2014.igem.org/wiki/images/4/40/BBa_K1364013.png

Source

This part results from the association of BBa_K823003, BBa_K1364002 and BBa_K1364003.

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BBa_K1364013/1