BBa_K1366021

BBa_K1366021 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1366021
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eduardo Cepeda Ca??edo
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:07

Genetic construct for lpp gen deletion



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1366021_sequence (Version 1)

Description

This biobrick contains the sequence homologies (50bp) to delete lpp gen (LPS moiety carrier) in E. coli with an ampicillin resistance and a Multiple Cloning Site (for the generation of genomic knock-ins of any desired gen in E. coli). The deletion of lpp gene and the replacement of that sequence with the Amp resistance. Gene deletions are performed with lambda-red recombination technology. FLP-FRT sequences are included to remove the antibiotic resistance with a specific recombinase.

Notes

This is the general structure
50bp homology Lpp // FRT // Amp R Promoter // RBS // AmpR // Terminator // FRT // MCS // 50 bp homology Lpp

Source

The genetic design is based in the lpp deletion done by:
Ni, Y., Reyes, J., & Chen, R. (2007). lpp Deletion as a Permeabilization Method. Biotechnology and Bioengineering, 1347-1356.
It also contains some parts of the registry like BBa_K1073000, BBa_B0034, BBa_B0015

Sequence Annotation Location Component / Role(s)
Lpp 50bp homology (1)
FRT
AmpR promoter
RBS
AmpR
Double terminator
FRT
Lpp 50pb homology (2)
MCS
1,50
51,84
85,113
114,126
127,982
983,1112
1113,1146
1217,1267
1147,1216
feature/misc sequence_feature
feature/dna sequence_feature
promoter feature/promoter
ribosome_entry_site feature/rbs
feature/cds CDS
stop_codon feature/stop
feature/dna sequence_feature
feature/misc sequence_feature
feature/scar
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1366021/1