C4

BBa_K1366103 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1366103

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Eduardo Cepeda Ca??edo, Mercedes Alejandra Vazquez Cantu

Date created: 2014-10-09 11:00:00

Date modified: 2015-05-08 01:10:07

GFP expression under a hTERT promoter (C4)

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1366103_sequence (Version 1)

Description

This composite act as a probe of concept of the transfection in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome. The GFP will be codon optimized for mammalian cell line.

Notes

The structure goes like this hTERT // RBS // GFP // Terminador

Source

We obtain the sequence of the hTERT promoter from the part BBa_K404106 and we used the GFP sequence that is available in uniprot and optimized it for mammalian cell expression

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
phTERT promoter	1,459	feature/promoter, promoter
BBa_K404106	1,459	engineered_region, feature/BioBrick
hGH terminator	1185,1666	sequence_feature, feature/misc
BBa_K404108	1185,1666	engineered_region, feature/BioBrick
Kozak sequence	460,472	ribosome_entry_site, feature/rbs
GFP	473,1184	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1366103/1

Member of these Collections

• iGEM Parts Registry